DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CFTR and MRP

DIOPT Version :9

Sequence 1:	NP_000483.3	Gene:	CFTR / 1080	HGNCID:	1884	Length:	1480	Species:	Homo sapiens
Sequence 2:	NP_723772.2	Gene:	MRP / 34686	FlyBaseID:	FBgn0032456	Length:	1549	Species:	Drosophila melanogaster

Alignment Length:	1507	Identity:	389/1507 - (25%)
Similarity:	692/1507 - (45%)	Gaps:	266/1507 - (17%)

- Green bases have known domain annotations that are detailed below.

Human     5 PLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKN--- 66
            |...||.:|::.:.|...:..||||..||..|::.:...||...:.......|::.:  :||
  Fly   225 PELSASFLSRITYQWFDKMALKGYRNPLEEKDLWDLRPQDSCSEVMPIFAHHWNQNV--RKNYKN 287

Human    67 -----PK--------------------------LINALRRCFFWRFMFYGIFLYLGEVTKAVQPL 100
                 ||                          ::..:.:.|...|:|..:.....:.....||.
  Fly   288 KARVEPKAQFSNGNVTFENPHGEKNGRKKGMASIMPPIYKSFGGVFLFGALMKLFTDTLTFAQPQ 352

Human   101 LLGRIIASYDPDNKEERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTL 165
            :|..||:..:..:.|.....|...:.|.:|...:|.:|......:..:|:::|.|:.:.||:|.|
  Fly   353 VLSLIISFVEAQDAEPEWKGILYAVLLFVLAAAQTFILGQYFHRMFIVGLRIRTALINAIYRKAL 417

Human   166 KLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFL 230
            ::|:....:.::|::|:|::.:..:|.|.....:.:|.||||:.|.:..:|:.|..|...||..:
  Fly   418 RISNSTKKESTVGEIVNLMAVDAQRFMELTTYLNMIWSAPLQIGLALYFLWQQLGPSVLAGLAVM 482

Human   231 IVLALFQAGLGRMM-------MKYRDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENL 288
            |:|......:...:       |||:|       ||:.:.:|::..|:.:|.|.||.:.||.:.::
  Fly   483 IILIPVNGVIASRIKTYQIRQMKYKD-------ERVKLMNEVLSGIKVLKLYAWEPSFEKQVLDI 540

Human   289 RQTELKLTRKAAYVRYFNSSAFFFSGFFVVFLSVLPYAL---IKGIILRKIFTTISFCIVLRMAV 350
            |..|:...|..||:....|..:..:.|.|..::...|.|   ...:.:.|:..:|:...::::.:
  Fly   541 RDKEIATLRSTAYLNAGTSFLWSCAPFLVSLVTFATYVLTSEANQLSVEKVLVSIALFDLMKLPL 605

Human   351 TRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVVMENVTAFWEEGFGELFEKAK-- 413
            | ..|.......::..::|:|..||..:|   |:.|                   ..|.:.:|
  Fly   606 T-ILPMLSVDIAETQVSVNRINKFLNSEE---LDPN-------------------SVLHDSSKPH 647

Human   414 -QNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEP 477
             .:..|.:.|.||:.            .|::||.::::|.|:|:.|:.|:||:|::...:||:|.
  Fly   648 PMSIENGEFSWGDEI------------TLRNINIEVKKGSLVALVGTVGSGKSSVVQAFLGEMEK 700

Human   478 SEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLG 542
            ..|.:...|::::..|.:||...|:::||:||.:||..||..||.||.|..||...:..|...:|
  Fly   701 LAGVVNTVGKLAYVPQQAWIQNATVRDNILFGQTYDRKRYNKVIDACALRADIDILSAGDLTEIG 765

Human   543 EGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCK--LMANKTRILVTS 605
            |.||.|||||:.|||||||||.||||||||.|...:|....|.|||..:..  ::|.|:|:|||.
  Fly   766 EKGINLSGGQKQRISLARAVYSDADLYLLDDPLSAVDAHVGKHIFEEVIGPKGILARKSRVLVTH 830

Human   606 KMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFS 670
            .:..|.:.|.|.::..|.....|||.:|...:..|:..::
  Fly   831 GVTFLPQVDSIYVIKMGEISESGTFDQLVKNKGAFADFII------------------------- 870

Human   671 LEGDAPVSWTETKKQSFKQTGEFGEKRKNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEP---- 731
                           ...|.|...|:..|.|...|:|                   .:|.|
  Fly   871 ---------------QHLQEGNEEEEELNQIKRQISS-------------------TADVPELLG 901

Human   732 -LERRLSLVPDSEQGEAILPRISVISTGPTLQARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVS 795
             :|:.:.|.    :.|::...|||.|..            :||    ..|.::.|:|.......|
  Fly   902 TVEKAIKLA----RTESLSDSISVTSAD------------SLM----GGGGSLRRRTKRQDSHDS 946

Human   796 LAPQANL-----TELDIYSRRLSQETGLEISEEINEEDLKECFFDDMESIPAVTTWNTYLRYITV 855
            :|..|:|     .|..:.....||..|:|.:                          .|..||
  Fly   947 VASAASLKKKQEVEGKLIETEKSQTGGVEFA--------------------------VYKHYI-- 983

Human   856 HKSL-IFVLIWCLV---IFLA-EVAASLVVLWLLGNTPLQDKGNSTHSRNNSYAVIITSTSSYYV 915
             ||: ||:.:..||   :|.| ::.::   |||......|:..|.|..|:
  Fly   984 -KSVGIFLSVATLVLNFVFQAFQIGSN---LWLTQWANDQNVANDTGLRD--------------- 1029

Human   916 FYIYVGVADTLLAMGFFRGLPLVHTLITV-------SKILHHKMLHSVLQAPMST-LNTLKAGGI 972
              :|:||..   |.||.:|:.....::.|       :|.:|:::|..:::..:.. .:....|.:
  Fly  1030 --MYLGVYG---AFGFGQGVLAYFAVVIVYLGGFQAAKTIHNELLAVIIRGSVCRFFDITPIGRL 1089

Human   973 LNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLRAYFLQTS 1037
            ||.||.|:.::|:.||.|:..|:..:.:|:..|.|:::..|......||:...:...:.:::.||
  Fly  1090 LNSFSGDMDVVDEELPATMDSFMTFIFMVLATIVVISLSTPIFLAVIVPIAFLYYFAQRFYVATS 1154

Human  1038 QQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMR 1102
            :||.:|||..||||::|...::.|..|:||:.....|.......::.:....:..:...||..:|
  Fly  1155 RQLMRLESVSRSPIYSHFSETVTGASTIRAYNVGDRFIEESDAKVDKNQVCKYPSVIANRWLAIR 1219

Human  1103 IEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRVFKFI 1167
            :||:..:..:..:..::|......|.||:.::.|:.:..||.|.|..|.|:::.:.||.|:.::.
  Fly  1220 LEMVGNLIILFASLFAVLGGQTNPGLVGLSVSYALQVTQTLNWLVRMSSDIETNIVSVERIKEYG 1284

Human  1168 DMPTEGKPTKSTKPYKNGQLSKVMIIENSHVKKDDIWPSGGQMTVKDLTAKYTEGGNAILENISF 1232
            :       ||...|::         :|....|..: ||..|::..::...:|.||.:.:|..:||
  Fly  1285 E-------TKQEAPWE---------LEQDKNKPKN-WPQEGRVEFQNFQVRYREGLDLVLRGVSF 1332

Human  1233 SISPGQRVGLLGRTGSGKSTLLSAFLRLLNTE-GEIQIDGVSWDSITLQQWRKAFGVIPQKVFIF 1296
            :|..|::||::||||:|||:|..|..|::... |.|.||||...|:.|...|....:|||...:|
  Fly  1333 NIQGGEKVGIVGRTGAGKSSLTLALFRIIEAAGGRISIDGVDIASMGLHMLRSRLTIIPQDPVLF 1397

Human  1297 SGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVL 1361
            ||:.|.||||:|..:|.||||..:...|:|.::.....|:..:.:||..||.|.:||:||||::|
  Fly  1398 SGSLRINLDPFEIKTDDEIWKALELSHLKSFVKSLAAGLNHEIAEGGENLSVGQRQLVCLARALL 1462

Human  1362 SKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDS 1426
            .|.|:|:|||.:|.:|..|..:|::|::..|.:|||:...||:..:|:..:.:|:::.::.::.|
  Fly  1463 RKTKVLVLDEATAAVDLETDDLIQKTIRTEFKECTVLTIAHRLNTILDSDKVIVLDKGQIIEFAS 1527

Human  1427 IQKLL-NERSLF 1437
            ..:|| |.:|.|
  Fly  1528 PTELLDNPKSAF 1539

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CFTR	NP_000483.3	CFTR_protein	1..1480	CDD:273530	389/1507 (26%)
		ABC_membrane	85..303	CDD:279056	56/224 (25%)
		ABCC_CFTR1	389..670	CDD:213258	89/285 (31%)
		CFTR_R	639..849	CDD:291094	31/219 (14%)
		Disordered R region. /evidence=ECO:0000269\|PubMed:10792060	654..831		30/186 (16%)
		ABC_membrane	866..1147	CDD:279056	69/292 (24%)
		ABCC_CFTR2	1208..1480	CDD:213256	87/232 (38%)
		Interaction with GORASP2. /evidence=ECO:0000269\|PubMed:21884936	1386..1480		15/53 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1452..1480
		PDZ-binding. /evidence=ECO:0000269\|PubMed:11707463, ECO:0000269\|PubMed:16331976	1478..1480
MRP	NP_723772.2	MRP_assoc_pro	14..1548	CDD:188098	389/1507 (26%)
		ABC_membrane	338..601	CDD:279056	63/269 (23%)
		ABCC_MRP_domain1	649..848	CDD:213217	81/210 (39%)
		ABC_membrane	988..1260	CDD:279056	68/294 (23%)
		ABCC_MRP_domain2	1308..1528	CDD:213211	81/219 (37%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165151590
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	5	4.750