Protein Alignment ERN2 and IRE1

DIOPT Version :10

• Sequence 1: NP_150296.4
• Gene: ERN2 / 10595
• HGNCID: 16942
• Length: 926
• Species: Homo sapiens
• Sequence 2: NP_011946.1
• Gene: IRE1 / 856478
• SGDID: S000001121
• Length: 1115
• Species: Saccharomyces cerevisiae
• Alignment Length: 1084
• Identity: 301/1084 - (27%)
• Similarity: 460/1084 - (42%)
• Gaps: 296/1084 - (27%)

Human    32 VHTLRPENLLLVSTLDGSLHALSKQTGDLKWTLRDD---PVIE----GPMYVTEMAFLSDPADGS 89
            ::.|...::|:.:.::|.|||:.::.|.:.|::..:   |:||    ..:...|...:....||:
Yeast   114 LNELSLSDILIAADVEGGLHAVDRRNGHIIWSIEPENFQPLIEIQEPSRLETYETLIIEPFGDGN 178

Human    90 LYILGTQKQQGLMKLPFTIPELVHASPCR-------------SSDGVFYTGRKQDAWFVVDPESG 141
            :|....  .|||.|||.:|.:||..||..             ..|...|||..:...:.::..:|
Yeast   179 IYYFNA--HQGLQKLPLSIRQLVSTSPLHLKTNIVVNDSGKIVEDEKVYTGSMRTIMYTINMLNG 241

Human   142 ETQMTLTTEGPSTPRLYIGRTQYTVTMHDPRAPALRWNTTYRRYSAPPMDGSPGKYMSHLASCGM 206
            |   .::..||.:...|.|                          :..:|.||.:.:. |..|..
Yeast   242 E---IISAFGPGSKNGYFG--------------------------SQSVDCSPEEKIK-LQECEN 276

Human   207 GLLL----------TVDPGSGTV---LWTQD-LGVPVMGVYTWHQDGLRQLP--HLTLARDTLHF 255
            .:::          :.|..|..|   .|.|: |.||:....|:.:||:...|  ..:|....|.|
Yeast   277 MIVIGKTIFELGIHSYDGASYNVTYSTWQQNVLDVPLALQNTFSKDGMCIAPFRDKSLLASDLDF 341

Human   256 LALRWGHIRLPASGPRDTATLFSTLDTQLLMTLYVGKDETGFYVSKALVHTGVALVPRGLTLAPA 320
            ...||.....|.                    :.||.    |.|...|......|||........
Yeast   342 RIARWVSPTFPG--------------------IIVGL----FDVFNDLRTNENILVPHPFNPGDH 382

Human   321 DGPTTDEVTL-QVSG------EREGSPSTAVRYPSGSVALPSQW--------------LLIGHHE 364
            :..::::|.| |.|.      ..:..||.....|....|...:|              .::|.|:
Yeast   383 ESISSNKVYLDQTSNLSWFALSSQNFPSLVESAPISRYASSDRWRVSSIFEDETLFKNAIMGVHQ 447

Human   365 L------------------------PPVL-----HTTMLRVHPTLGSGTAETRPPENTQAPAFFL 400
            :                        ||::     .||.|..:..:.| ..|...||:.:|
Yeast   448 IYNNEYDHLYENYEKTNSLDTTHKYPPLMIDSSVDTTDLHQNNEMNS-LKEYMSPEDLEA----- 506

Human   401 ELLSLSREKLWDS---ELHPEEKTPDSYLGLGPQDLLAASL------TAVLLGGWILF------- 449
                 .|:|:.:.   ||  :||..:|.|      |...||      |.|.|..:::|
Yeast   507 -----YRKKIHEQISREL--DEKNQNSLL------LKFGSLVYRIIETGVFLLLFLIFCAILQRF 558

Human   450 --------------VMRQQQPQVVEKQ-------QETPLAPADFAHISQ---------------- 477
                          .|.:::..:||.:       .|....|.|.....|
Yeast   559 KILPPLYVLLSKIGFMPEKEIPIVESKSLNCPSSSENVTKPFDMKSGKQVVFEGAVNDGSLKSEK 623

Human   478 --------DAQSLHSGASRRSQKRLQSPSKQAQ-------PLDDPEAEQLTVVGKISFNPKDVLG 527
                    |.:||.....::.:||.....|:.:       |..:...:.|.|..||       ||
Yeast   624 DNDDADEDDEKSLDLTTEKKKRKRGSRGGKKGRKSRIANIPNFEQSLKNLVVSEKI-------LG 681

Human   528 RGAGGTFVFRGQFEGRAVAVKRLLRECFGLVRREVQLLQESDRHPNVLRYFCTERGPQFHYIALE 592
            .|:.||.||:|.|:||.|||||:|.:...:...|::||.|||.||||:||:|:|...:|.|||||
Yeast   682 YGSSGTVVFQGSFQGRPVAVKRMLIDFCDIALMEIKLLTESDDHPNVIRYYCSETTDRFLYIALE 746

Human   593 LCRASLQEYVENPDLDRGGLE------PEVVLQQLMSGLAHLHSLHIVHRDLKPGNILI------ 645
            ||..:||:.||:.::....|:      |..:|:|:.||:||||||.|:||||||.|||:
Yeast   747 LCNLNLQDLVESKNVSDENLKLQKEYNPISLLRQIASGVAHLHSLKIIHRDLKPQNILVSTSSRF 811

Human   646 -----TGPDSQGLGRVVLSDFGLCKKLPAGRCSFSLHSGIP-GTEGWMAPELLQ----------- 693
                 ||.::.   |:::|||||||||.:|:.||..:...| ||.||.|||||:
Yeast   812 TADQQTGAENL---RILISDFGLCKKLDSGQSSFRTNLNNPSGTSGWRAPELLEESNNLQCQVET 873

Human   694 --------LLPPDS---P------TSAVDIFSAGCVFYYVLSGGSHPFGDSLYRQANILTGAPCL 741
                    ::..||   |      |.::||||.||||||:||.|.|||||...|::||:.|   :
Yeast   874 EHSSSRHTVVSSDSFYDPFTKRRLTRSIDIFSMGCVFYYILSKGKHPFGDKYSRESNIIRG---I 935

Human   742 AHLEEE--VHDKVV---ARDLVGAMLSPLPQPRPSAPQVLAHPFFWSRAKQLQFFQDVSDWLEKE 801
            ..|:|.  :||:.:   |.||:..|:...|..||:|.:||.||.||.::|:|:|...|||.||.|
Yeast   936 FSLDEMKCLHDRSLIAEATDLISQMIDHDPLKRPTAMKVLRHPLFWPKSKKLEFLLKVSDRLEIE 1000

Human   802 SEQEP--LVRALEAGGCAVV-RDNWHEHISMPLQTDLRKFRSYKGTSVRDLLRAVRNKKHHYREL 863
            :...|  |:...:||...|: ..:|..........:|.::|.|..:.:.|||||:|||.||:.:|
Yeast  1001 NRDPPSALLMKFDAGSDFVIPSGDWTVKFDKTFMDNLERYRKYHSSKLMDLLRALRNKYHHFMDL 1065

Human   864 PVEVRQALGQVPDGFVQYFTNRFPRLLLHTHRAMRSCASESLFL 907
            |.::.:.:|.|||||..|||.|||.||:..:..::...|:...|
Yeast  1066 PEDIAELMGPVPDGFYDYFTKRFPNLLIGVYMIVKENLSDDQIL 1109

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ERN2	NP_150296.4	Luminal_IRE1	38..366	CDD:188875	77/408 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	478..509		7/37 (19%)
		STKc_IRE1	517..782	CDD:270884	134/315 (43%)
		RNase_Ire1	785..910	CDD:199217	46/126 (37%)
IRE1	NP_011946.1	Luminal_IRE1	120..407	CDD:188875	70/342 (20%)
		STKc_IRE1	672..981	CDD:270884	136/321 (42%)
		RNase_Ire1	984..1113	CDD:199217	46/126 (37%)