Protein Alignment PAK4 and SKM1

DIOPT Version :10

• Sequence 1: NP_005875.1
• Gene: PAK4 / 10298
• HGNCID: 16059
• Length: 591
• Species: Homo sapiens
• Sequence 2: NP_014528.1
• Gene: SKM1 / 854036
• SGDID: S000005473
• Length: 655
• Species: Saccharomyces cerevisiae
• Alignment Length: 633
• Identity: 172/633 - (27%)
• Similarity: 277/633 - (43%)
• Gaps: 169/633 - (26%)

Human    11 ISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESA-------RRPKPLVDPACITSIQPGAPK 68
            :|:|:||.|:||.|||.....|.|:|..|..|::.|.       |.                 .|
Yeast   123 VSSPTNFTHKVHVGFDPKVGNFVGVPDSWAKLLQTSEITYDDWNRN-----------------SK 170

Human    69 TIVRGSKGAKD--GALTLLLDEFENMSV--------------TRSNSLRRDSPPPPARARQENGM 117
            .:::..:..:|  |..|:..::..|.|:              .|:||::|.......:.:.::.:
Yeast   171 AVIKALQFYEDYNGLDTMQFNDHLNTSLDLKPLKSPTRYIINKRTNSIKRSVSRTLRKGKTDSIL 235

Human   118 PEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSG 182
            |...:                                  |.:|
Yeast   236 PVYQS----------------------------------ELKP---------------------- 244

Human   183 PDVGTPQPAGLASGAKLAAGRPFNTYPR-ADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSS 246
                                     :|| :|.|:  :....|.:.|...|      .:..|..|:
Yeast   245 -------------------------FPRPSDDDY--KFTNIEDNKVREEG------RVHVSKEST 276

Human   247 SRPPTRARGAPSPGV----LGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREP------QRVSH 301
            :...|:..|.....|    |..|.:.....|....|:|||...........:|.      ...|:
Yeast   277 ADSQTKQLGKKEQKVIQSHLRRHDNNSTFRPHRLAPSAPATKNHDSKTKWHKEDLLELKNNDDSN 341

Human   302 EQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIA-----------------TVRSSGKLVAV 349
            |.......:.:|. :||.|.....|.|:|::|.|.::                 ..|..|:.||:
Yeast   342 EIIMKMKTVAIDV-NPRPYFQLVEKAGQGASGAVYLSKRIKLPQENDPRFLKSHCHRVVGERVAI 405

Human   350 KKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGD-ELWVVMEFLEGGALTDIV------ 407
            |::.|.:|.:::|:.||:::|.|.:.||:|....:|::.| ||||:||::|||.||||:
Yeast   406 KQIRLSEQPKKQLIMNELLVMNDSRQENIVNFLEAYIIDDEELWVIMEYMEGGCLTDILDAVARS 470

Human   408 ---THTR-MNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKE 468
               .|:. :||.|:|.:.....|.|..||.:.:||||||||:|||...|.||::|||||.:::::
Yeast   471 NTGEHSSPLNENQMAYIVKETCQGLKFLHNKKIIHRDIKSDNILLNSQGLVKITDFGFCVELTEK 535

Human   469 VPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPP 533
            ..:|.::||||||||||::::..|..:||:||||||:|||::|||||.||.||||:.:|.:|..|
Yeast   536 RSKRATMVGTPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSP 600

Human   534 RLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASI 581
            :|::...||...|.|||..|..:...||:..:||...||:.|..|..:
Yeast   601 KLRHPESVSKQTKQFLDACLQVNVESRASVRKLLTFEFLSMACSPEQL 648

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PAK4	NP_005875.1	CRIB_PAK_like	10..55	CDD:238526	17/50 (34%)
		Linker	25..320		47/328 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	95..301		27/216 (13%)
		GEF-interaction domain (GID)	298..323		6/24 (25%)
		STKc_PAK4	300..591	CDD:132988	122/310 (39%)
SKM1	NP_014528.1	PH_Cla4_Ste20	4..113	CDD:270097
		PBD	122..180	CDD:395634	18/73 (25%)
		STKc_PAK	359..640	CDD:270789	114/280 (41%)