TRiP-CRISPR toolbox fly stocks
- Gal4+VPR (tissue-specific gene overexpression)
- Gal4+Cas9 (for tissue specific gene KO)
- nanos-Cas9 (for gene KO in germline)
Cross Gal4+dCas9-VPR stocks to sgRNA stocks from the TRiP-OE VPR collection (sgRNAs cloned into pCFD4) to overexpress target gene.
GAL4/UAS expression of Cas9 proteins with dead nuclease activity (dCas9), fused to VPR transcriptional activator (dCas9-VPR).
Not compatible with TRiP-OE sgRNA stocks from the flySAM collections (sgRNAs cloned into U6BsgRNA2.0 or flySAM2.0).
Gene activation - cross to TRiP-OE sgRNA stocks (VPR colletion) |
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Nickname | Availability | Genotype |
elav>VPR | in progress | P{w[+mW.hs]=GawB}elav[C155];P{UAS-3XFLAG-dCas9-VPR}attp40/CyO |
MS1096>VPR | 67039 | w[1118] P{w[+mW.hs]=GawB}Bx[MS1096];P{UAS-3XFLAG-dCas9-VPR}attp40/CyO |
pnr>VPR | 67040 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/(TM6B,Tb[1]) |
dMef2 > VPR | 67041 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1]) |
C96 > VPR | 67042 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GawB}bbg[C96]/TM6B,Tb[1] |
Lpp > VPR | 67043 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{Lpp-GAL4.B}/TM6B,Tb[1] |
Mhc > VPR | 67044 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{[ry+]MHC-82-Gal4}/TM6B, Tb[1] |
dpp > VPR | 67045 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}TM6B,Tb[1] |
hh > VPR | 67046 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; hh-Gal4 /TM6B,Tb[1] |
Delta[ts] > VPR | in progress | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{w[+mW.hs]=GawB}Dl[05151] ,P{w[+mC]=tubP-GAL80[ts]}[*] /TM6B,Tb[1] |
tubulin > VPR | in progress | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; tub-Gal4 /TM6B,Tb[1] |
tubulin > VPR | 67048 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40; tub-Gal4 / SM5;TM6B Tb[1] |
en > VPR | 67049 | w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/ CyO;UASP{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
spalt > VPR | 67050 | w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
blistered > VPR | 67051 | w[*]; P{w[+mC]=bs-GAL4.Term}G1/ CyO;UAS:P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
nos > VPR | 67052 | w[*]; P{w[+mC]=GAL4-nos.NGT}40 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
twi > VPR | 67053 | w[*]; P{w[+mC]=GAL4-twi.2xPE}1 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
esg > VPR + GFP | 67054 | w[*];esg-Gal4,P{ UAS-GFP} / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
nubbin > VPR | 67055 | w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
3.1Lsp2 > VPR | in progress | w[*]; P{w{[+mC]=Gal4-Lsp2-3.1} / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
Myo1A > VPR | 67057 | w[*]; P{GawB}Myo31DFNP0001/ CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] |
Temporal control of gene activation - cross to TRiP-OE sgRNA stocks (VPR colletion) |
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Nickname | Availability | Genotype |
elav[ts] > VPR | 67058 | P{w[+mW.hs]=GawB}elav[C155]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO |
Lpp[ts] > VPR | 67061 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO;P{Lpp-GAL4.B}/TM6B,Tb[1] |
Mhc[ts] > VPR | 67062 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO; P{[ry+]MHC-82-Gal4}/TM6B, Tb[1] |
dMef2[ts] > VPR | 67063 | w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1]) |
Myo1A[ts] > VPR | 67067 | w[*]; P{GawB}Myo31DFNP0001/CyO; P{UAS-3XFLAG-dCas9-VPR}attp2, P{w[+mC]=tubP-GAL80[ts]}2/TM6B,Tb[1] |
3.1Lsp2 > VPR | in progress | w[*]; P{w{[+mC]=Gal4-Lsp2-3.1}/CyO; P{UAS-3XFLAG-dCas9-VPR}attp2, P{w[+mC]=tubP-GAL80[ts]}2/TM6B,Tb[1] |
bs[ts]> VPR | 67071 | w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 |
en[ts]> VPR | 67069 | w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 |
spalt[ts] > VPR | 67070 | w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 |
dpp[ts]> VPR | 67066 | w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}40C.6/TM6B, Tb[1] |
C96[ts] > VPR | 67064 | w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mW.hs]=GawB}bbg[C96] |
MS1096[ts]>VPR | 67059 | w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10 |
Dl[ts]> VPR | 67047 | w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GawB}Dl[05151-G], P{w[+mC]=tubP-GAL80[ts]}2/TM6B, Tb[1] |
pnr[ts]>VPR | 67060 | w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/TM6B, Tb[1] |
tubulin > VPR | 67065 | w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1] |
esg[ts]> VPR | 67072 | w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+*]=UAS-GFP.U}2; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 |
Cross Gal4+Cas9 stocks to sgRNA stocks from the TRiP-KO collection (sgRNAs cloned into pCFD3 or pCFD4) to cut target gene.
Somatic tissue-specific GAL4/UAS expression of Cas9
Gene cutting/knockout in somatic cells to make mutant tissue - cross to TRiP-KO sgRNA stocks |
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Nickname | Availability | Genotype |
elav > Cas9 | 67073 | P{w[+mW.hs]=GawB}elav[C155]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO |
ms1096 > Cas9 | 67074 | w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO |
dMef2 > Cas9 | 67075 | w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1]) |
C96 > Cas9 | 67076 | w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}bbg[C96]/TM6B,Tb[1] |
Pannier > Cas9 | 67077 | w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/(TM6B,Tb[1]) |
Lpp > Cas9 | in progress | w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{Lpp-GAL4.B}/TM6B,Tb[1] |
Mhc > Cas9 | 67079 | w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{[ry+]MHC-82-Gal4}/TM6B, Tb[1] |
actin > Cas9 | in progress | w[*]; P{w[+mC]=Act5C-GAL4}25FO1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |
en > Cas9 | in progress | w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |
spalt > Cas9 | 67081 | w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |
blistered > Cas9 | 67082 | w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |
twi > Cas9 | 67084 | w[*]; P{w[+mC]=GAL4-twi.2xPE}1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |
esg > Cas9 + GFP | 67085 | w[*];esg-Gal4,P{ UAS-GFP}/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B |
nubbin > Cas9 | 67086 | w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |
3.1Lsp2 > Cas9 | 67087 | w[*]; P{w{[+mC]=Gal4-Lsp2-3.1}/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B |
Myo1A > Cas9 | 67088 | w[*]; P{GawB}Myo31DFNP0001/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |
Cross nanos+Cas9 stocks to sgRNA stocks from the TRiP-KO collection (sgRNAs cloned into pCFD3 or pCFD4) to cut target gene.
germline-specific GAL4/UAS expression of Cas9 to make mutant animals
Gene cutting/knockout in somatic cells to make mutant tissue - cross to TRiP-KO sgRNA stocks |
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Nickname | Availability | Genotype |
nos-Cas9 | in progress | y[1] sc[*] v[1]; P{Nos-Cas9}Attp40 |
nos-Cas9 | in progress | y[1] sc[*] v[1]; P{Nos-Cas9}Attp2 |
nos > Cas9 | 67083 | w[*]; P{w[+mC]=GAL4-nos.NGT}40/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu |