The TRiP facility at Harvard Medical School maintains the entire collection of TRiP stocks for people wishing to screen the lines en masse.

The facility is located on the third floor of the New Research Building (NRB) on the Harvard Medical School campus, in space contiguous with the DRSC. The TRiP screening facility provides the space and equipment to carry out a complete survey of the current set of TRiP stocks. If you are interested to screen, please contact the TRiP Team at TRiP@flyrnai.org.

All stocks generated in the TRiP are available at the Screening Center. This includes all of the lines available at BDSC (lines generated in the VALIUM10 vector) and approximately 600 stocks lines generated in the VALIUM1 vector. For maximal knock-down using the TRiP lines made in VALIUM1, it is best that your GAL4 also contain UAS-dicer2.

The TRiP team is currently generating about 250 transgenes per month. As they are ready, the corresponding fly lines will be sent to the BDSC for distribution.

1. Contact TRiP Screening Center to reserve a screening space. At this time you will need to discuss specifics of the your screen so that the TRiP Team will be able to meet your screening needs. Items to consider include any special reagents and/or equipment needed, method of phenotypic readout, etc.

   Three to four months before arriving at the TRiP Screening Center:

2. If screening TRiP stocks in the VALIUM1 vector, prepare your GAL4 line in a UAS-dcr2 background. At present approximately 600 TRiP stocks are in the weaker of the TRiP vectors (VALIUM1). To achieve maximum knock down your GAL4 line should also carry UAS-dcr2. It may take up to 2 months to construct your GAL4; UAS-dcr2 stock so you should plan for this event ahead of the time your TRiP screen will start.

   Two months before arriving at the TRiP Screening Center:

3. Send all fly stocks the screener will require for screening (e.g., GAL4 lines) to the TRiP Screening Center. Once these stocks arrive at HMS members of the TRiP team will put the lines through quarantine (1 month required) as well as amplify them (1 month required). This will ensure that the screener will be able to initiate their screen immediately upon their arrival at the screening center.

Please Note: All stocks brought into the TRiP Facility and Screen Center are required to go through quarantine! No exceptions!

While the cost for screening this collection at HMS has not been determined, a close estimate will be $2.50 (US dollars) per line. By far the greatest expense will be traveling to and living in Boston.

Note: If needed, members of the TRiP will be available to answer questions and provide suggestions for possible housing options in Boston. However, it is the responsibility of the screener to arrange for housing.