Transgenic RNAi Resource Project

TRiP >> TRiP Reagents

Please refer to our Approach for information on vector design. Features of the following vectors are listed in the downloadable pdf files.

Knockdown Vectors

pVALIUM10 (GU931382)
map and sequence
 Cloning Protocol
for 1st Generation VALIUM knockdown vectors
pWALIUM10 (GU931388)
map and sequence
pVALIUM1 (GU931383)
map and sequence
pVALIUM20 (Accession ID to come)
map and sequence
 Cloning Protocol
for 2nd Generation VALIUM knockdown vectors
pVALIUM22 (Accession ID to come)
map and sequence
pWALIUM20 (Accession ID to come)
map and sequence
pWALIUM22 (Accession ID to come)
map and sequence
 

Overexpression Vectors

pVALIUM10-roe (GU931386)
map and sequence
Cloning Protocol
pVALIUM10-moe (GU931387)
map and sequence
Cloning Protocol
pWALIUM10-roe (Accession ID to come)
map and sequence
Cloning Protocol
pWALIUM10-moe (GU931385)
map and sequence
Cloning Protocol
 

Cloning Tools

mENTRY (GU931384)
map and sequence
Cloning Protocol
 

 

If use of any of the TRiP reagents results in data that is included in a manuscript for publication, the TRiP requests that a version of the following statement be included in the Acknowledgements section: "We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing plasmid vectors used in this study."

 •  To request TRiP reagents please contact us at TRiP@flyrnai.org.

 •  For vector-specific questions please contact JianQuan Ni jni@genetics.med.harvard.edu.