The following is a list of all TRiP stocks that have been generated thus far. It includes lines made in vectors that work in the soma (VALIUM1, VALIUM10, VALIUM20) and lines made in vectors that work in the germline (VALIUM20, VALIUM22, VALIUM21). This list will be updated as new stocks are added to the collection.
Almost all of the TRiP transgenic fly stocks in the VALIUM10 and VALIUM1 vectors are available at the Bloomington Drosophila Stock Center. Newly generated stocks, including those made in the VALIUM20, VALIUM21 and VALIUM22 vectors, are being sent to BDSC on a regular basis.
To learn more about the vector and hairpin design for the TRiP RNAi lines please read here.
Also visit our FAQ page.
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Please Note: All TRiP stocks should have a wildtype appearance as the wildtype vermillion gene is used as the selection marker for the hairpin transformants.
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If use of any of the TRiP stocks results in data that is included in a manuscript for publication, the TRiP requests that a version of the following statement be included in the Acknowledgements section: "We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing transgenic RNAi fly stocks used in this study."
The TRiP welcomes feedback on the lines you have received. In particular, we appreciate information relevant to the efficiency and specificity of knockdown (e.g., whether or not an expected phenotype is observed). This information will allow us to troubleshoot and plan for future RNAi lines. Please send feedback to TRiP@flyrnai.org.
