Sub-Libraries for RNAi Knockdown of Functionally Related Gene Sets

In response to community demand, we have constructed dsRNA sub-libraries that target genes grouped by biological and/or biochemical function.

• Rapid screening (fewer plates to screen than with the full genomic collection)
• Curated gene lists drawn from the published literature, known and predicted functions, and shared by experts in their fields
• Deeper coverage-multiple unique dsRNA amplicons per gene
• Adheres to current rules for minimizing off-target effects
• Available for on-site screening or in experiment-ready 384-well assay plates for screening at your institution (contact the Director for details)

Currently Available Sub-Libraries

More on the Currently Available Sub-Libraries

Drosophila Kinase/Phosphatase RNAi Sub-Library (DRSC KP). This sub-library targets the complete set of Drosophila genes that encode proteins with kinase or phosphatase activity. Approximately three amplicon per gene coverage. 563 genes. Eight assay plates.

Drosophila Transcription Factor RNAi Sub-Library (DRSC TRXN). This sub-library targets the complete set of Drosophila genes that encode transcription factors. Approximately two amplicon per gene coverage. 993 genes. Nine assay plates.

Drosophila Ubiquitin-Related Genes RNAi Sub-Library (NYU-DRSC UBIQ). Drosophila Ubiquitin-Related Genes RNAi Sub-Library (NYU-DRSC UBIQ). This sub-library targets genes involved in ubiquitination. The sub-set was generated by our collaborators at New York University's RNAi Core Facility and is available at the DRSC for on-site screening only.

What next? New sub-sets are planned based on community input. Please contact the Director with ideas.