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DRSC
>> Reagents
>> RNAi Sub-Libraries
Sub-Libraries for RNAi Knockdown of Functionally Related Gene Sets
In response to community demand, we have constructed dsRNA sub-libraries that target genes grouped by biological and/or biochemical function.
Features include:
- Rapid screening (fewer plates to screen than with the full genomic collection)
- Curated gene lists drawn from the published literature, known and predicted functions, and shared by experts in their fields
- Deeper coverage-multiple unique dsRNA amplicons per gene
- Adheres to current rules for minimizing off-target effects
- Available for on-site screening or in experiment-ready 384-well assay plates for screening at your institution (contact the Director for details)
Currently Available Sub-Libraries
| Library |
Gene Set |
# of Genes |
Ave. # of amplicons per gene |
# of Assay Plates |
Gene List |
| DRSC KP |
Kinases & Phosphatases |
563 |
3 |
8 |
gene list |
| DRSC TRXN |
TFs, Nuclear & DNA Binders |
993 |
2 |
9 |
gene list |
| NYU-DRSC UBIQ |
Ubiquitin-Related Genes |
439 |
2-3 |
6 |
gene list |
| NYU-DRSC TM |
Transmembrane Genes |
1729 |
2 |
17 |
gene list |
More on the Currently Available Sub-Libraries
Drosophila Kinase/Phosphatase RNAi Sub-Library (DRSC KP). This sub-library targets Drosophila genes that encode proteins for kinase or phosphatase. Approximately three amplicon per gene coverage. 563 genes. Eight assay plates. Amplicons targeting both kinase and phosphatase genes are distributed randomly on all eight plates.
Layout of empty wells (aqua) and LacZ controls (purple) on an
experiment-ready plate from the KP sub-library. Similar layouts for
the other sub-libraries.
Drosophila Transcription Factors (and Related) RNAi Sub-Library (DRSC TRXN). This sub-library targets the complete set of Drosophila genes that encode transcription factors as well as other DNA binding proteins and nuclear factors. Approximately two amplicon per gene coverage. 993 genes. Nine assay plates.
Drosophila Ubiquitin-Related Genes RNAi Sub-Library (NYU-DRSC UBIQ). This sub-library targets genes involved in ubiquitination. The sub-set was generated by our collaborators at New York University's RNAi Core Facility and is available at the DRSC for on-site screening only.
Drosophila Transmembrane Genes RNAi Sub-Library (NYU-DRSC TM). This sub-library targets genes annotated as transmembrane proteins or computationally predicted as such. The sub-set was generated by our collaborators at New York University's RNAi Core Facility and is available at the DRSC for on-site screening only.
What next? New sub-sets are planned based on community input. Please contact the Director with ideas.
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