DRSC
>> Screens
>> Completed Screens By Topic
Screens for genes relevant to chromosome pairing
Bateman JR, Larschan E, D'Souza R, Marshall
LS, Dempsey KE, Johnson JE, Mellone BG, Kuroda MI. A
Genome-Wide Screen Identifies Genes That Affect Somatic
Homolog Pairing in Drosophila. G3 July 1, 2012 vol. 2
no. 7 731-740.
Joyce EF, Williams BR, Xie T, Wu
CT. Identification of Genes That Promote or Antagonize
Somatic Homolog Pairing Using a High-Throughput FISH-Based
Screen. PLoS Genet. 2012 May;8(5):e1002667. Epub 2012
May 10.
Screens in Primary Cells
Schulte J, Sepp KJ, Wu C, Hong P, Littleton
JT. High-Content Chemical and RNAi Screens for
Suppressors of Neurotoxicity in a Huntington's Disease
Model. PLoS ONE 2011;6(8):e23841. Epub 2011 Aug 31.
Perrimon N, Zirin J, Bai J. Primary Cell
Cultures from Drosophila Gastrula Embryos. J Vis
Exp. 2011 Feb 28;(48). pii: 2215. doi: 10.3791/2215.
Wu C, Schulte J, Sepp KJ, Littleton JT, Hong
P. Automatic robust neurite detection and morphological
analysis of neuronal cell cultures in high-content
screening. Neuroinformatics. 2010 Jun;8(2):83-100.
Sepp KJ, Hong P, Lizarraga SB, Liu JS, Mejia
LA, Walsh CA, Perrimon N. Identification of neural outgrowth
genes using genome-wide RNAi. PLoS Genet 2008 Jul
4;4(7):e1000111.
Bai J, Binari R, Ni JQ, Vijayakanthan M, Li
HS, Perrimon N. RNA interference screening in Drosophila
primary cells for genes involved in muscle assembly and
maintenance. Development. 2008 Apr;135(8):1439-49.
Screens for genes relevant to Cell Viability, Metabolism
and Cell Death
Kondo S, Perrimon N. A Genome-Wide RNAi
Screen Identifies Core Components of the G2-M DNA Damage
Checkpoint. Sci. Signal. 2011 Jan 4. Vol. 4, Issue
154, p. rs1
Ravi D, Wiles AM, Bhavani S, Ruan J, Leder
P, Bishop AJ. A network of conserved damage survival
pathways revealed by a genomic RNAi screen. PLoS
Genet 2009 Jun;5(6)
Correction
Kwon M, Godinho SA, Chandhok NS, Ganem NJ,
Azioune A, Thery M, Pellman D. Mechanisms to suppress
multipolar divisions in cancer cells with extra
centrosomes. Genes Dev. 2008 Aug 15;22(16):2189-203.
Epub 2008 Jul 28.
Farny NG, Hurt JA, Silver PA. Definition of
global and transcript-specific mRNA export pathways in
metazoans. Genes Dev. 2008 Jan 1;22(1):66-78.
Yi CH, Sogah DK, Boyce M, Degterev A,
Christofferson DE, Yuan J. A genome-wide RNAi screen reveals
multiple regulators of caspase activation. J Cell
Biol. 2007 Nov 19;179(4):619-26.
Screens for genes relevant to Transcription, Translation
& RNA Biology
DRSC Reagents screened at
the NYU RNAi
Core: Imberg-Kazdan K, Ha S, Greenfield A, Poultney
CS, Bonneau R, Logan SK, Garabedian MJ. A genome-wide RNA
interference screen identifies new regulators of androgen
receptor function in prostate cancer cells. Genome
Res. 2013 Apr;23(4):581-91. doi:
10.1101/gr.144774.112. Epub 2013 Feb 12.
Stender JD, Pascual G, Liu W, Kaikkonen MU,
Do K, Spann NJ, Boutros M, Perrimon N, Rosenfeld MG,
Glass CK. Control of Proinflammatory Gene Programs by
Regulated Trimethylation and Demethylation of Histone
H4K20. Molecular Cell, 23 August 2012,
10.1016/j.molcel.2012.07.020.
Larschan E, Soruco MM, Lee OK, Peng S,
Bishop E, Chery J, Goebel K, Feng J, Park PJ, Kuroda
MI. Identification of Chromatin-Associated Regulators of MSL
Complex Targeting in Drosophila Dosage Compensation. PLoS
Genet. 2012 Jul;8(7):e1002830. Epub 2012 Jul 26.
Grimberg KB, Beskow A, Lundin D, Davis MM,
Young P. Basic-Leucine Zipper Protein Cnc-C is a
Substrate and Transcriptional Regulator of the Drosophila
26S Proteasome. Mol Cell Biol. 2011
Feb;31(4):897-909. Epub 2010 Dec 13.
Mourikis P, Lake RJ, Firnhaber CB, Dedecker
BS. Modifiers of notch transcriptional activity identified
by genome-wide RNAi. BMC Dev Biol. 2010 Oct
19;10(1):107.
Dekanty A, Romero NM, Bertolin AP, Thomas
MG, Leishman CC, Perez-Perri JI, Boccaccio GL, Wappner
P. Drosophila genome-wide RNAi screen identifies multiple
regulators of HIF-dependent transcription in
hypoxia. PLoS Genet. 2010 Jun 24;6(6):e1000994.
Zhou R, Hotta I, Denli AM, Hong P, Perrimon
N, Hannon GJ. Comparative Analysis of Argonaute-Dependent
Small RNA Pathways in Drosophila, Molecular Cell, Volume 32,
Issue 4, 21 November 2008, Pages 592-599.
Wagner EJ, Burch BD, Godfrey AC, Salzler HR,
Duronio RJ, Marzluff WF. A genome-wide RNA interference
screen reveals that variant histones are necessary for
replication-dependent histone pre-mRNA processing. Mol
Cell. 2007 Nov 30;28(4):692-9.
Lu J, Ruhf ML, Perrimon N, Leder P. A
genome-wide RNA interference screen identifies putative
chromatin regulators essential for E2F repression. Proc
Natl Acad Sci U S A. 2007 May 29;104(22):9381-6.
Cherry S, Doukas T, Armknecht S, Whelan S,
Wang H, Sarnow P, Perrimon N. Genome-wide RNAi screen
reveals a specific sensitivity of IRES-containing RNA
viruses to host translation inhibition. Genes
Dev. 2005 Feb 15;19(4):445-52.
Screens for genes relevant to Response to Pathogen
Infection
Stender JD, Pascual G, Liu W, Kaikkonen MU,
Do K, Spann NJ, Boutros M, Perrimon N, Rosenfeld MG,
Glass CK. Control of Proinflammatory Gene Programs by
Regulated Trimethylation and Demethylation of Histone
H4K20. Molecular Cell, 23 August 2012,
10.1016/j.molcel.2012.07.020.
Moser TS, Jones RG, Thompson CB, Coyne CB,
Cherry S. A kinome RNAi screen identified AMPK as promoting
poxvirus entry through the control of actin
dynamics. PLoS Pathog. 2010 Jun 17;6(6):e1000954.
Akimana C, Al-Khodor S, Abu Kwaik Y. Host
Factors Required for Modulation of Phagosome Biogenesis and
Proliferation of Francisella tularensis within the
Cytosol. PLoS One. 2010 Jun 11;5(6):e11025.
Sessions OM, Barrows NJ, Souza-Neto JA,
Robinson TJ, Hershey CL, Rodgers MA, Ramirez JL,
Dimopoulos G, Yang PL, Pearson JL, Garcia-Blanco MA.
Discovery of insect and human dengue virus host
factors. Nature 2009 Apr 23; 485; 1047-50.
Philips JA, Porto MC, Wang H, Rubin EJ,
Perrimon N. ESCRT factors restrict mycobacterial
growth. Proc Natl Acad Sci U S A. 2008 Feb
26;105(8):3070-5.
Derré I, Pypaert M, Dautry-Varsat A, Agaisse
H. RNAi screen in Drosophila cells reveals the involvement
of the Tom complex in Chlamydia infection. PLoS
Pathog. 2007 Oct 26;3(10):1446-58.
Agaisse H, Burrack LS, Philips JA, Rubin EJ,
Perrimon N, Higgins DE. Genome-wide RNAi screen for host
factors required for intracellular bacterial
infection. Science. 2005 Aug
19;309(5738):1248-51.
Philips JA, Rubin EJ, Perrimon N. Drosophila
RNAi screen reveals CD36 family member required for
mycobacterial infection. Science. 2005 Aug
19;309(5738):1251-3.
Cherry S, Doukas T, Armknecht S, Whelan S,
Wang H, Sarnow P, Perrimon N. Genome-wide RNAi screen
reveals a specific sensitivity of IRES-containing RNA
viruses to host translation inhibition. Genes
Dev. 2005 Feb 15;19(4):445-52.
Cherry S, Kunte A, Wang H, Coyne C, Rawson
RB, Perrimon N. COPI activity coupled with fatty acid
biosynthesis is required for viral replication. PLoS
Pathog. 2006 Oct;2(10):e102.
Screens for genes relevant to Neurobiology
and Neurodegenerative Disease
Schulte J, Sepp KJ, Wu C, Hong P, Littleton
JT. High-Content Chemical and RNAi Screens for
Suppressors of Neurotoxicity in a Huntington's Disease
Model. PLoS ONE 2011;6(8):e23841. Epub 2011 Aug 31.
Wu C, Schulte J, Sepp KJ, Littleton JT, Hong
P. Automatic robust neurite detection and morphological
analysis of neuronal cell cultures in high-content
screening. Neuroinformatics. 2010 Jun;8(2):83-100.
Zhang S, Binari R, Zhou R, Perrimon N. A
genomewide RNA interference screen for modifiers of
aggregates formation by mutant Huntingtin in
Drosophila. Genetics. 2010 Apr;184(4):1165-79. Epub
2010 Jan 25.
Sepp KJ, Hong P, Lizarraga SB, Liu JS, Mejia
LA, Walsh CA, Perrimon N. Identification of neural outgrowth
genes using genome-wide RNAi. PLoS Genet 2008 Jul
4;4(7):e1000111.
Screens for genes relevant to Signal Transduction
Bergwitz C, Wee MJ, Sinha S, Huang J,
DeRobertis C, Mensah LB, Cohen J, Friedman A, Kulkarni M, Hu
Y, Vinayagam A, Schnall-Levin M, Berger B, Perkins LA, Mohr
SE, Perrimon N. Genetic Determinants of Phosphate Response
in Drosophila. PLoS ONE 2013;8(3):e56753. doi: 10.1371/journal.pone.0056753. Epub 2013 Mar 8.
Stender JD, Pascual G, Liu W, Kaikkonen MU,
Do K, Spann NJ, Boutros M, Perrimon N, Rosenfeld MG,
Glass CK. Control of Proinflammatory Gene Programs by
Regulated Trimethylation and Demethylation of Histone
H4K20. Molecular Cell, 23 August 2012,
10.1016/j.molcel.2012.07.020.
Aikin R, Cervantes A, D'Angelo G, Ruel L,
Lacas-Gervais S, Schaub S, Thérond P. A Genome-Wide
RNAi Screen Identifies Regulators of Cholesterol-Modified
Hedgehog Secretion in Drosophila. PLoS
One. 2012;7(3):e33665. Epub 2012 Mar 14.
Friedman A, Tucker G, Singh R, Yan D,
Vinayagam A, Hu Y, Binari R, Hong P, Sun X, Porto M,
Pacifico S, Murali T, Finley R, Asara JM, Berger B, Perrimon
N. Proteomic and functional genomic landscape of receptor
tyrosine kinase and Ras to extracellular signal-regulated
kinase signaling. Science Signaling. 2011 Oct
25;4(196):rs10.
Port F, Hausmann G, Basler K. A genome-wide
RNA interference screen uncovers two p24 proteins as
regulators of Wingless secretion. EMBO Rep. 2011, doi:
10.1038/embor.2011.165.
Booker M, Samsonova AA, Kwon Y, Flockhart I,
Mohr SE, Perrimon N. False negative rates in Drosophila
cell-based RNAi screens: A case study. BMC Genomics
2011, 12:50doi:10.1186/1471-2164-12-50.
Kondo S, Perrimon N. A Genome-Wide RNAi
Screen Identifies Core Components of the G2-M DNA Damage
Checkpoint. Sci. Signal. 2011 Jan 4. Vol. 4, Issue
154, p. rs1
Mourikis P, Lake RJ, Firnhaber CB, Dedecker
BS. Modifiers of notch transcriptional activity identified
by genome-wide RNAi. BMC Dev Biol. 2010 Oct
19;10(1):107.
Moser TS, Jones RG, Thompson CB, Coyne CB,
Cherry S. A kinome RNAi screen identified AMPK as promoting
poxvirus entry through the control of actin
dynamics. PLoS Pathog. 2010 Jun 17;6(6):e1000954.
Kockel L, Kerr KS, Melnick M, Brückner
K, Hebrok M, Perrimon N. Dynamic Switch of Negative
Feedback Regulation in Drosophila Akt-TOR
Signaling. PLoS Genet 2010 Jun 17;6(6):e1000990.
Nir O, Bakal C, Perrimon N, Berger B.
Inference of RhoGAP/GTPase regulation using single-cell
morphological data from a combinatorial RNAi screen.
Genome Research. Mar;20(3):372-80. Epub 2010 Feb 9.
Bakal C, Linding R, Llense F, Heffern E,
Martin-Blanco E, Pawson T, Perrimon N. Phosphorylation
networks regulating JNK activity in diverse genetic
backgrounds. Science 2008 Oct 17;322(5900):453-6.
Sathyanarayanan S, Zheng X, Kumar S, Chen
CH, Chen D, Hay B, Sehgal A. Identification of novel genes
involved in light-dependent CRY degradation through a
genome-wide RNAi screen. Genes Dev. 2008 Jun
1;22(11):1522-33.
Wang J, Zhou X, Bradley PL, Chang SF,
Perrimon N, Wong ST. Cellular phenotype recognition for
high-content RNA interference genome-wide screening. J
Biomol Screen. 2008 Feb;13(1):29-39.
Xu L, Yao X, Chen X, Lu P, Zhang B, Ip
YT. Msk is required for nuclear import of
TGF-{beta}/BMP-activated Smads. J Cell Biol. 2007 Sep
10;178(6):981-94.
Bakal C, Aach J, Church G, Perrimon
N. Quantitative morphological signatures define local
signaling networks regulating cell
morphology. Science. 2007 Jun
22;316(5832):1753-6.
Gwack Y, Srikanth S, Feske S, Cruz-Guilloty
F, Oh-hora M, Neems DS, Hogan PG, Rao A. Biochemical and
functional characterization of Orai proteins. J Biol
Chem. 2007 Jun 1;282(22):16232-43. Epub 2007 Feb 9.
Friedman A, Perrimon N. A functional RNAi
screen for regulators of receptor tyrosine kinase and ERK
signalling. Nature. 2006 Nov 9;444(7116):230-4.
Zhang SL, Yeromin AV, Zhang XH, Yu Y,
Safrina O, Penna A, Roos J, Stauderman KA, Cahalan
MD. Genome-wide RNAi screen of Ca(2+) influx identifies
genes that regulate Ca(2+) release-activated Ca(2+) channel
activity. Proc Natl Acad Sci U S A. 2006 Jun
13;103(24):9357-62.
Gwack Y, Sharma S, Nardone J, Tanasa B, Iuga
A, Srikanth S, Okamura H, Bolton D, Feske S, Hogan PG, Rao
A. A genome-wide Drosophila RNAi screen identifies
DYRK-family kinases as regulators of
NFAT. Nature. 2006 Jun 1;441(7093):646-50.
Vig M, Peinelt C, Beck A, Koomoa DL, Rabah
D, Koblan-Huberson M, Kraft S, Turner H, Fleig A, Penner R,
Kinet JP. CRACM1 is a plasma membrane protein essential for
store-operated Ca2+ entry. Science. 2006 May
26;312(5777):1220-3.
Feske S, Gwack Y, Prakriya M, Srikanth S,
Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M, Rao A. A
mutation in Orai1 causes immune deficiency by abrogating
CRAC channel function. Nature. 2006 May
11;441(7090):179-85
Nybakken K, Vokes SA, Lin TY, McMahon AP,
Perrimon N. A genome-wide RNA interference screen in
Drosophila melanogaster cells for new components of the Hh
signaling pathway. Nat Genet. 2005
Dec;37(12):1323-32.
Baeg GH, Zhou R, Perrimon N. Genome-wide
RNAi analysis of JAK/STAT signaling components in
Drosophila. Genes Dev. 2005 Aug
15;19(16):1861-70.
DasGupta R, Kaykas A, Moon RT, Perrimon
N. Functional genomic analysis of the Wnt-wingless signaling
pathway. Science. 2005 May 6;308(5723):826-33.
Screens for genes relevant to Import/Export,
Influx/Efflux and Transport
Bergwitz C, Wee MJ, Sinha S, Huang J,
DeRobertis C, Mensah LB, Cohen J, Friedman A, Kulkarni M, Hu
Y, Vinayagam A, Schnall-Levin M, Berger B, Perkins LA, Mohr
SE, Perrimon N. Genetic Determinants of Phosphate Response
in Drosophila. PLoS ONE 2013;8(3):e56753. doi: 10.1371/journal.pone.0056753. Epub 2013 Mar 8.
Stotz SC, Clapham DE. Anion-sensitive
fluorophore identifies the Drosophila swell-activated
chloride channel in a genome-wide RNA interference
screen. PLoS One. 2012;7(10):e46865. doi:
10.1371/journal.pone.0046865. Epub 2012 Oct 4.
Aikin R, Cervantes A, D'Angelo G, Ruel L,
Lacas-Gervais S, Schaub S, Thérond P. A Genome-Wide
RNAi Screen Identifies Regulators of Cholesterol-Modified
Hedgehog Secretion in Drosophila. PLoS
One. 2012;7(3):e33665. Epub 2012 Mar 14.
Port F, Hausmann G, Basler K. A genome-wide
RNA interference screen uncovers two p24 proteins as
regulators of Wingless secretion. EMBO Rep. 2011, doi:
10.1038/embor.2011.165.
Wendler F, Gillingham AK, Sinka R,
Rosa-Ferreira C, Gordon DE, Franch-Marro X, Peden AA,
Vincent JP, Munro S. A genome-wide RNA interference screen
identifies two novel components of the metazoan secretory
pathway. EMBO J. 2010 Jan 20;29(2):304-14. Epub 2009 Nov 26.
Jiang D, Zhao L, Clapham DE. Genome-wide
RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+
antiporter. Science. 2009 Oct 2;326(5949):144-7.
Cuttell L, Vaughan A, Silva E, Escaron CJ,
Lavine M, Van Goethem E, Eid JP, Quirin M, Franc NC.
Undertaker, a Drosophila Junctophilin, links Draper-mediated
phagocytosis and calcium homeostasis. Cell. 2008 Oct
31;135(3):524-34.
Xu L, Yao X, Chen X, Lu P, Zhang B, Ip
YT. Msk is required for nuclear import of
TGF-{beta}/BMP-activated Smads. J Cell Biol. 2007 Sep
10;178(6):981-94.
Gwack Y, Srikanth S, Feske S, Cruz-Guilloty
F, Oh-hora M, Neems DS, Hogan PG, Rao A. Biochemical and
functional characterization of Orai proteins. J Biol
Chem. 2007 Jun 1;282(22):16232-43. Epub 2007 Feb 9.
Zhang SL, Yeromin AV, Zhang XH, Yu Y,
Safrina O, Penna A, Roos J, Stauderman KA, Cahalan
MD. Genome-wide RNAi screen of Ca(2+) influx identifies
genes that regulate Ca(2+) release-activated Ca(2+) channel
activity. Proc Natl Acad Sci U S A. 2006 Jun
13;103(24):9357-62.
Vig M, Peinelt C, Beck A, Koomoa DL, Rabah
D, Koblan-Huberson M, Kraft S, Turner H, Fleig A, Penner R,
Kinet JP. CRACM1 is a plasma membrane protein essential for
store-operated Ca2+ entry. Science. 2006 May
26;312(5777):1220-3.
Feske S, Gwack Y, Prakriya M, Srikanth S,
Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M, Rao A. A
mutation in Orai1 causes immune deficiency by abrogating
CRAC channel function. Nature. 2006 May
11;441(7090):179-85
Bard F, Casano L, Mallabiabarrena A, Wallace
E, Saito K, Kitayama H, Guizzunti G, Hu Y, Wendler F,
Dasgupta R, Perrimon N, Malhotra V. Functional genomics
reveals genes involved in protein secretion and Golgi
organization. Nature. 2006 Feb 2;439(7076):604-7.
Screens for genes relevant to Cell Division,
Morphology and the Cytoskeleton
Bateman JR, Larschan E, D'Souza R, Marshall
LS, Dempsey KE, Johnson JE, Mellone BG, Kuroda MI. A
Genome-Wide Screen Identifies Genes That Affect Somatic
Homolog Pairing in Drosophila. G3 July 1, 2012 vol. 2
no. 7 731-740.
Joyce EF, Williams BR, Xie T, Wu
CT. Identification of Genes That Promote or Antagonize
Somatic Homolog Pairing Using a High-Throughput FISH-Based
Screen. PLoS Genet. 2012 May;8(5):e1002667. Epub 2012
May 10.
Aikin R, Cervantes A, D'Angelo G, Ruel L,
Lacas-Gervais S, Schaub S, Thérond P. A Genome-Wide
RNAi Screen Identifies Regulators of Cholesterol-Modified
Hedgehog Secretion in Drosophila. PLoS
One. 2012;7(3):e33665. Epub 2012 Mar 14.
Rohn JL, Sims D, Liu T, Fedorova M, Schoeck
F, Dopie J, Vartiainen M, Kiger AA, Perrimon N, Baum B.
Comparative RNAi screening identifies a conserved core
metazoan actinome by phenotype. J. Cell Biol. 2011,
194. 789-805.
Wu C, Schulte J, Sepp KJ, Littleton JT, Hong
P. Automatic robust neurite detection and morphological
analysis of neuronal cell cultures in high-content
screening. Neuroinformatics. 2010 Jun;8(2):83-100.
Moser TS, Jones RG, Thompson CB, Coyne CB,
Cherry S. A kinome RNAi screen identified AMPK as promoting
poxvirus entry through the control of actin
dynamics. PLoS Pathog. 2010 Jun 17;6(6):e1000954.
Nir O, Bakal C, Perrimon N, Berger B.
Inference of RhoGAP/GTPase regulation using single-cell
morphological data from a combinatorial RNAi screen.
Genome Research. Mar;20(3):372-80. Epub 2010 Feb 9.
Erhardt S, Mellone BG, Betts CM, Zhang W,
Karpen GH, Straight AF.
Genome-wide analysis reveals a cell cycle-dependent mechanism
controlling centromere propagation.
J Cell Biol. 2008 Dec 1;183(5):805-18.
Sims D, Duchek P, Baum B. PDGF/VEGF
signalling controls cell size in Drosophila. Genome
Biol. 2009 Feb 12;10(2):R20.
Kwon M, Godinho SA, Chandhok NS, Ganem NJ,
Azioune A, Thery M, Pellman D. Mechanisms to suppress
multipolar divisions in cancer cells with extra
centrosomes. Genes Dev. 2008 Aug 15;22(16):2189-203.
Epub 2008 Jul 28.
Sepp KJ, Hong P, Lizarraga SB, Liu JS, Mejia
LA, Walsh CA, Perrimon N. Identification of neural outgrowth
genes using genome-wide RNAi. PLoS Genet 2008 Jul
4;4(7):e1000111.
Bai J, Binari R, Ni JQ, Vijayakanthan M, Li
HS, Perrimon N. RNA interference screening in Drosophila
primary cells for genes involved in muscle assembly and
maintenance. Development. 2008 Apr;135(8):1439-49.
Eggert US, Kiger AA, Richter C, Perlman ZE,
Perrimon N, Mitchison TJ, Field CM. Parallel chemical
genetic and genome-wide RNAi screens identify cytokinesis
inhibitors and targets. PLoS Biol. 2004
Dec;2(12):e379.
Boutros M, Kiger AA, Armknecht S, Kerr K,
Hild M, Koch B, Haas SA, Paro R, Perrimon N; Heidelberg Fly
Array Consortium. Genome-wide RNAi analysis of growth and
viability in Drosophila cells. Science. 2004 Feb
6;303(5659):832-5.
Kiger AA, Baum B, Jones S, Jones MR, Coulson
A, Echeverri C, Perrimon N. A functional genomic analysis of
cell morphology using RNA interference. J
Biol. 2003;2(4):27.
Screens for Genes Relevant to Organelle Formation,
Fission/Fusion and Function
Jiang D, Zhao L, Clapham DE. Genome-wide
RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+
antiporter. Science. 2009 Oct 2;326(5949):144-7.
Beller M, Sztalryd C, Southall N, Bell M,
Jäckle H, Auld DS, Oliver B. COPI Complex Is a
Regulator of Lipid Homeostasis. PLoS Biology 2008
Nov 25;6(11):e292.
Gandre-Babbe S and van der Bliek AM. The
Novel Tail-anchored Membrane Protein Mff Controls
Mitochondrial and Peroxisomal Fission in Mammalian
Cells. Mol Biol Cell 2008 Jun;19(6):2402-12. Epub
2008 Mar 19.
Screens Relevant to Circadian Rhythms
Sathyanarayanan S, Zheng X, Kumar S, Chen
CH, Chen D, Hay B, Sehgal A. Identification of novel genes
involved in light-dependent CRY degradation through a
genome-wide RNAi screen. Genes Dev. 2008 Jun
1;22(11):1522-33.
Screens Performed at NYU with DRSC Libraries
DRSC Reagents screened at
the NYU RNAi
Core: Imberg-Kazdan K, Ha S, Greenfield A, Poultney
CS, Bonneau R, Logan SK, Garabedian MJ. A genome-wide RNA
interference screen identifies new regulators of androgen
receptor function in prostate cancer cells. Genome
Res. 2013 Apr;23(4):581-91. doi:
10.1101/gr.144774.112. Epub 2013 Feb 12.
Bioinformatics and Data Analysis based on Screens done
at DRSC
Booker M, Samsonova AA, Kwon Y, Flockhart I,
Mohr SE, Perrimon N. False negative rates in Drosophila
cell-based RNAi screens: A case study. BMC Genomics
2011, 12:50doi:10.1186/1471-2164-12-50.
Wiles AM, Doderer M, Ruan J, Gu TT, Ravi D,
Blackman B, Bishop AJ. Building and analyzing protein
interactome networks by cross-species comparisons. BMC
Syst Biol. 2010 Mar 30;4(1):36.
Kaplow IM, Singh R, Friedman A, Bakal C,
Perrimon N, Berger
B. RNAiCut:
automated detection of significant genes from functional
genomic screens. Nat Methods. 2009 Jul;6(7):476-7.
Ravi D, Wiles AM, Bhavani S, Ruan J, Leder
P, Bishop AJ. A network of conserved damage survival
pathways revealed by a genomic RNAi screen. PLoS
Genet 2009 Jun;5(6)
Correction
Wiles AM, Ravi D, Bhavani S, Bishop AJ. An
Analysis of Normalization Methods for Drosophila RNAi
Genomic Screens and Development of a Robust Validation
Scheme. J Biomol Screen. 2008 Sep;13(8):777-84. Epub
2008 Aug 27.
Drosophila RNAi Methodology & Protocols
Perrimon N, Zirin J, Bai J. Primary Cell
Cultures from Drosophila Gastrula Embryos. J Vis
Exp. 2011 Feb 28;(48). pii: 2215. doi: 10.3791/2215.
Mohr S, Bakal C, Perrimon N. Genomic
Screening with RNAi: Results and Challenges. Annu Rev
Biochem. 2010;79:37-64.
Bai J, Sepp KJ, Perrimon N. Culture of
Drosophila primary cells dissociated from gastrula embryos
and their use in RNAi screening. Nat Protoc.
2009;4(10):1502-12. Epub 2009 Sep 24.
Wang J, Zhou X, Bradley PL, Chang SF,
Perrimon N, Wong ST. Cellular phenotype recognition for
high-content RNA interference genome-wide screening. J
Biomol Screen. 2008 Feb;13(1):29-39.
Ni JQ, Markstein M, Binari R, Pfeiffer B,
Liu LP, Villalta C, Booker M, Perkins L, Perrimon N. Vector
and parameters for targeted transgenic RNA interference in
Drosophila melanogaster. Nat Methods. 2008
Jan;5(1):49-51.
DasGupta R, Nybakken K, Booker M,
Mathey-Prevot B, Gonsalves F, Changkakoty B,Perrimon N. A
case study of the reproducibility of transcriptional
reporter cell-based RNAi screens in Drosophila. Genome
Biol. 2007;8(9):R203.
Ramadan N, Flockhart I, Booker M, Perrimon
N, Mathey-Prevot B. Design and implementation of
high-throughput RNAi screens in cultured Drosophila
cells. Nat Protoc. 2007;2(9):2245-64.
Perrimon N, Mathey-Prevot B. Applications of
high-throughput RNA interference screens to problems in cell
and developmental biology. Genetics. 2007
Jan;175(1):7-16.
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Hu Y, Flockhart I, Vinayagam A, Bergwitz C,
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