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DRSC
>> Screens
>> Publications from the DRSC
Publications from the DRSC (including methods, tools, off-target effects):
Perrimon N, Ni JQ, Perkins L. In vivo RNAi:
Today and Tomorrow. Cold Spring Harb Perspect Biol.
2010 Jun 9. [Epub ahead of print]
Mohr S, Bakal C, Perrimon N. Genomic
Screening with RNAi: Results and Challenges. Annu Rev
Biochem. 2010 Apr 1. [Epub ahead of print]
Bai J, Sepp KJ, Perrimon N. Culture of
Drosophila primary cells dissociated from gastrula embryos
and their use in RNAi screening. Nat Protoc.
2009;4(10):1502-12. Epub 2009 Sep 24.
Kondo S, Booker M, Perrimon N. Cross-species
RNAi Rescue Platform in Drosophila
melanogaster. Genetics. 2009 Aug 31. (Epub ahead of
print)
Kaplow IM, Singh R, Friedman A, Bakal C,
Perrimon N, Berger
B. RNAiCut:
automated detection of significant genes from functional
genomic screens. Nat Methods. 2009 Jul;6(7):476-7.
Ni JQ, Liu LP, Binari R, Hardy R, Shim HS,
Cavallaro A, Booker M, Pfeiffer B, Markstein M, Wang H,
Villalta C, Laverty T, Perkins L, Perrimon N. A Drosophila
Resource of Transgenic RNAi Lines for
Neurogenetics. Genetics. 2009; published ahead of
print on June 1, 2009 as doi: 10.1534/genetics.109.103630
Wang J, Zhou X, Bradley PL, Chang SF,
Perrimon N, Wong ST. Cellular phenotype recognition for
high-content RNA interference genome-wide screening. J
Biomol Screen. 2008 Feb;13(1):29-39.
Ni JQ, Markstein M, Binari R, Pfeiffer B,
Liu LP, Villalta C, Booker M, Perkins L, Perrimon N. Vector
and parameters for targeted transgenic RNA interference in
Drosophila melanogaster. Nat Methods. 2008
Jan;5(1):49-51.
DasGupta R, Nybakken K, Booker M,
Mathey-Prévôt B, Gonsalves F, Changkakoty B,Perrimon N. A
case study of the reproducibility of transcriptional
reporter cell-based RNAi screens in Drosophila. Genome
Biol. 2007;8(9):R203.
Ramadan N, Flockhart I, Booker M, Perrimon
N, Mathey-Prévôt B. Design and implementation of
high-throughput RNAi screens in cultured Drosophila
cells. Nat Protoc. 2007;2(9):2245-64.
Perrimon N, Mathey-Prévôt B. Applications of
high-throughput RNA interference screens to problems in cell
and developmental biology. Genetics. 2007
Jan;175(1):7-16.
Echeverri CJ, Beachy PA, Baum B, Boutros M,
Buchholz F, Chanda SK, Downward J, Ellenberg J, Fraser AG,
Hacohen N, Hahn WC, Jackson AL, Kiger A, Linsley PS, Lum L,
Ma Y, Mathey-Prévôt B, Root DE, Sabatini DM, Taipale J,
Perrimon N, Bernards R. Minimizing the risk of reporting
false positives in large-scale RNAi screens. Nat
Methods. 2006 Oct;3(10):777-9.
Kulkarni MM, Booker M, Silver SJ, Friedman
A, Hong P, Perrimon N, Mathey-Prévôt B. Evidence of
off-target effects associated with long dsRNAs in Drosophila
melanogaster cell-based assays. Nat Methods. 2006
Oct;3(10):833-8.
Echeverri CJ, Perrimon N. High-throughput
RNAi screening in cultured cells: a user's guide. Nat Rev
Genet. 2006 May;7(5):373-84.
Flockhart I, Booker M, Kiger A, Boutros M,
Armknecht S, Ramadan N, Richardson K, Xu A, Perrimon N,
Mathey-Prévôt B. FlyRNAi: the Drosophila RNAi screening
center database. Nucleic Acids Res. 2006 Jan
1;34(Database issue):D489-94
Armknecht S, Boutros M, Kiger A, Nybakken K,
Mathey-Prévôt B, Perrimon N. High-throughput RNA
interference screens in Drosophila tissue culture
cells. Methods Enzymol. 2005;392:55-73.
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