Drosophila RNAi Screening Center

DRSC >> Reagents >> RNAi Libraries

Genome-Wide Libraries for Drosophila RNAi Screening and Validation

The DRSC Genome-wide RNAi Library (DRSC 2.0)

The DRSC 2.0 is a collection of dsRNAs for genome-wide RNAi knockdown. It improves upon version 1.0 in several ways, most significantly in terms of minimizing the potential for off-target effects due to sequence similarity to genes other than the target. The library is regularly updated to accommodate newly identified genes and updated gene models.

Features include:
  • About 13,900 genes are represented in the collection (66 assay plates)
  • Average of one to two dsRNAs per gene
  • Coverage includes genes that encode proteins and non-coding RNAs
  • Genome-wide coverage lets you screen in an unbiased manner
  • Adheres to current rules for minimizing off-target effects
  • Most-used library at the DRSC, with many successful publications resulting from work on hits identified using this library

Availability. The DRSC 2.0 is available for on-site screening. In addition, a limited number of amplicons can be requested as PCR-amplified DNA

The DRSC Collection for Follow-Up with Distinct Reagents (DRSC Follow-Up)

DRSC Follow-Up is a collection of amplicons for RNAi knockdown of genes that have previously scored as hits in any screen at the DRSC.

As of spring 2008, DRSC Follow-Up covered about 6,000 amplicons. Amplicons directed against unrepresented genes are generated when new lists of screen hits submitted by screeners. Our ultimate goal is for genome-wide coverage.

Importantly, the DRSC Follow-Up amplicons are distinct from the amplicons in the 2.0 amplicons (i.e. target a different region of the gene), providing independent validation of a positive RNAi result. Please note that confirmation of the RNAi result does not eliminate the need for other follow-up, such as in vivo confirmation of biological relevance.

Availability. Screen hits can be requested as "cherry picks" from the DRSC Follow-Up collection. The requested amplicons are provided as PCR-amplified DNA templates. These can be used for dsRNA production at the home institution.