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DRSC
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>> High-Content Imaging
High-Content Imaging at the DRSC: Options for high-throughput, high-content imaging (non-confocal)
For some assays, confocal microscopy is either not necessary or not ideal. We provide several options for high-content imaging. Our microscopy experts can help you to decide which microscope will best fit with your assay.
Screeners at the DRSC can get access to fluorescence, non-confocal and light microscopes for high-throughput, high-content imaging at ICCB-Longwood, which is located in a building nearby the DRSC facility.
ICCB-L Imaging Information Page
ICCB-L List of high-content imaging equipment
High-Content Imaging at the DRSC: Molecular Devices Discovery-1 High-Throughput Fluorescence Microscope
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The Molecular Devices Discovery-1 is a fluorescence microscope (non-confocal) for high-content screening. The set-up at the DRSC is fully automated with a robotic (Thermo) and optimized for 384-well format. The Discovery-1 at DRSC was heavily used with good success at one time but is now showing its age. Please contact us for a current status report and view alternative options available at the ICCB (see above).
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Detailed Information about the Molecular Devices Discovery1
The Discovery-1 is set up for fluorescence microscopy (10x or 20x lens, non-confocal) with full automation for 384-well plates.
Why would I choose the Discovery-1?
Discovery-1 data files are compatible with the popular image analysis software package MetaMorph. It has been used successfully for a number of years for screening with fluorescent markers such as GFP or antibodies.
Why would I not choose the Discovery-1?
If you have antibodies against a protein of interest and you are more interested in relative protein levels than in sub-cellular localization of the protein, the LiCor Aerius would be the better choice (alternatively, LiCor and high-content imaging can be combined).
If you are interested in features such as sub-cellular localization that require confocal analysis, the Opera confocal fluorescent microscope would be the better choice.
Software. Images can be downloaded and analyzed off-line of the microscope system using Molecular Devices' MetaMorph software. In addition, images can be exported in other formats for analysis with other software tools.
Data Storage. Bring an external hard-drive (200 GB or more) for data storage.
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