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DRSC
>> About Us
>> Screening Costs
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Note on Costs from the Director, April 2011.
NIH has asked us to begin recovering a larger
percentage of our budget from fees. This is similar to
models at other screening centers. We keep costs down
through cost-efficiency, including improved protocols,
a small staff, bulk discounts, etc. Nevertheless,
costs involved in reagent production, maintaining
state-of-the-art equipment, etc. are high. We are
happy to help estimate costs for grant
applications. When appropriate we will provide letters
of support for grant applications, including travel
grants for visiting screeners. Ultimately we do not
want cost alone to stand in the way of a strong
screening project, so please contact the Director if
you feel you are in that position and we will work
with you to try to make screening possible.
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Please see below for information about library fees and
other costs (consumables, travel and lodging in Boston for
visiting screeners, etc.) that you will need to
anticipate.
DRSC Fees (Updated April 2011)
In all cases, the cost of shipping materials for off-site
screening is additional (typically, we ask for a FedEx
account number so shipping is billed directly to your
institution). Also, we will have to add the cost of the
assay plates if you are using specialized imaging plates
such as those required for the Perkin Elmer Evotec
Opera.
Screening Libraries
DRSC "2.0" Full-Genome Library (one screen in
duplicate; on-site or off-site screening; 384-well format
only): $6500.00
DRSC
Sub-Libraries (one screen in duplicate; on-site of
off-site screening; 384-well format only; Transmembane
library excluded): $750.00
DRSC Transmembrane Sub-Library (one screen in duplicate;
on-site or off-site screening; 384-well format only):
$1500.00
Custom Library based on your gene list and our existing
amplicons, Per 96-well Plate (enough dsRNA for many
screens; on-site or off-site screening; includes a quality
analysis and the PCR cherry-pick plate for later
re-synthesis): $1200.00 per 96-well Plate
Over-Expression Library (one screen in duplicate; on-site
screening only; 384-well format): $2000.00.
Access to additional
Library Resources, such as Small Molecule
Libraries and Mammalian RNAi Reagents
Please note that the DRSC can help you gain access to
small-molecule libraries for concurrent RNAi and
small-molecule screening on-site if that is of interest to
you. Please contact the Director for more information or
see
the ICCB-Longwood
Screening Facility for information about their small
molecule libraries. We can also put you in touch with
mammalian screening facilities for concurrent screening in
mammalian cells or for follow-up on fly screen hits in
mammalian cells.
Reagents for Assay Development and
Optimization
Sample Cell Lines: No charge (shipping costs only)
Set of Four Test Plates (on-site or off-site screening;
384-well format only): $250.00
Automated Individual Selection ("Cherry-Pick")
of PCR Amplicons for dsRNA production by you: $4.00 per
amplicon
Reagents for Screen Follow-Up
Automated Individual Selection ("Cherry-Pick")
of PCR Amplicons for dsRNA production by you: $4.00 per
amplicon
Custom Library based on your gene list and our existing
amplicons (see above)
Opera Fees (high-throughput, high-content
fluorescence confocal imaging)
Definitions: Screen user, an active screener at the DRSC
or ICCB-L (in assay development, assay optimization, active
screening or secondary screening stage). Non-screen user,
anyone else
Opera Image Acquisition Fees: One-time training
fee, all users, $250.00. Hourly fee, screen users, $12.00
per hour. Hourly fee, non-screen users, $20.00 per
hour. Maximum Opera usage fee for DRSC full-genome screeners
will be $3500.00 (in addition to applicable DRSC screening
fees) for the primary screening portion of the project and
first round of secondary screening/follow-up
cherry-picks.
Additional Costs Involved in High-Throughput
Screening
Consumable Costs. The costs of cell culture media, sera,
wash buffers, luciferase reagents, antibodies, fluorescent
dyes, dsRNA for single-gene knockdown in all wells in
addition to a library, etc. as well as consumables such as
robot pipet tips and Opera compatible plates can add up
fast. Please contact us to help estimate costs of your
screen for planning or grant application purposes.
Travel and Lodging. For researchers
visiting from afar, the cost of travel and lodging of the
visiting screener is an additional cost that is absorbed by
the group doing the screen. Please see
our information
page about staying in Boston or contact the Director for
more information.
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